Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica

first_imgWith the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the thermal process of oil degradation is expensive and ineffective. Thus, in this study an indigenous cold-adapted Antarctic soil bacterium, Rhodococcus erythropolis strain AQ5-07, was screened for biosurfactant production ability using the multiple approaches of blood haemolysis, surface tension, emulsification index, oil spreading, drop collapse and “MATH” assay for cellular hydrophobicity. The growth kinetics of the bacterium containing different canola oil concentration was studied. The strain showed β-haemolysis on blood agar with a high emulsification index and low surface tension value of 91.5% and 25.14 mN/m, respectively. Of the models tested, the Haldane model provided the best description of the growth kinetics, although several models were similar in performance. Parameters obtained from the modelling were the maximum specific growth rate (qmax), concentration of substrate at the half maximum specific growth rate, Ks% (v/v) and the inhibition constant Ki% (v/v), with values of 0.142 h−1, 7.743% (v/v) and 0.399% (v/v), respectively. These biological coefficients are useful in predicting growth conditions for batch studies, and also relevant to “in field” bioremediation strategies where the concentration of oil might need to be diluted to non-toxic levels prior to remediation. Biosurfactants can also have application in enhanced oil recovery (EOR) under different environmental conditions.last_img read more

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Big Catch

first_imgI am, quite possibly, the world’s worst fly fisherman. A lack of patience and complete technical incompetence have combined within me to create a sort of natural repellent to native and non-native trout species. I’m heavy-footed, loud and can tie only one knot: the kind that comes untied as soon as you tug on it. What’s that knot called again? Anyway, fish usually elude me, and yet, I love the practice of rejection. Standing ankle deep in cold water, the repetitive motion of the cast, the quiet of the creek…even my profanity has a rhythm to it, bursting forth every minute and a half when I get my line stuck in rhodo. Ah, fly fishing. I need all the help I can get. Thankfully, SweetWater Brewing Company has my back. They’ve created a new program centered around their limited edition fish/puzzle cans that helps stock local streams with trout. Because really, fishing is a numbers game. The more fish in that river, the better chance I’ll have at tricking one of them to come home with me for dinner.Show Sweetwater a picture of your stacked fish cans on one of those handy social media platforms and they’ll stock a trout into a local stream. It’s a cool program that has anglers from all over the Southeast posting pics of their sick cans. Even President Jimmy Carter has gotten in on the action, posting his own picture with a stack of fishy Sweetwater IPAs in his hands. Carter actually played a significant part in my development as a horrible fisherman. While he was president, he established the Chattahoochee River National Recreation Area. Shortly after that designation, I learned to fish on that river. Back then, the ‘Hooch was over-crowded with tubers and over-polluted by assholes, but I vividly remember landing a massive rainbow on one of my first outings. I was using a cheap spin rod that my dad set up for me. He had a smallmouth bass that he caught before I was born mounted and hung on the wall of his office. As my father and I reeled the fish in together, my 8-year-old mind immediately starting clearing space in my own room for this new fish that would certainly be trophy size. It would be a glorious, great-white-hunter kind of trophy. I’d probably start wearing those safari-style khaki vests and carrying a knife. That’s what great fishermen do, after all. The fish crested the water as we pulled it close. My father knelt down, grabbing the massive trout with his left hand and wrestling the hook loose with his right. It was massive. I started to wonder how long it would take to mount a fish. Could we have it done today? So, I could take it to school on Monday? Of course, as soon as the fish was free from the hook, it slipped from my dad’s hands. Turns out, I come from a long line of really bad fishermen. If we had a family crest, it would be of a middle-aged man swearing at a fish as it swims away. Being such a poor angler stings. But these new cans from Sweetwater help. Would it be weird if I mounted them on the wall in my office? #fishforAFishlast_img read more

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Police Constable, friend injured in motorcycle accident

first_imgAn allegedly drunken driver found himself in hot water after the minibus he owns and operates collided with a motorcycle, seriously injuring a Police Officer and his pillion rider.The motorcycle (CK 461) which Police Constable Azad Fredericks was riding, along with his friend, Daniel Mondesir, at the time of the accidentThe injured men were identified as 22-year-old Police Constable Azad Fredericks, who is attached to the Alberttown Police Station, Georgetown, and 29-year-old Daniel Mondesir of Salem Village, Parika, East Bank Essequibo (EBE).The minibus owner and driver, 62-year-old Lachminayan Ramnarine, of Lot 26 A Anna Catherina, West Coast Demerara (WCD), is presently in Police custody, assisting with the investigation.Reports are that on Saturday about 19:45h, Ramnarine was driving along “Bus Shed Street”, Anna Catherina, when he allegedly switched lanes, causing his vehicle to collide with the motorcycle.As a result of the impact of the collision, both Fredericks and Mondesir were thrown from the motorcycle and landed on the roadway. They sustained multiple injuries about their bodies.Guyana Times was told that the injured men were picked up by public-spirited persons and taken to the Leonora Cottage Hospital, WCD, where the doctor on duty immediately transferred them to the West Demerara Regional Hospital (WDRH) after examining their injuries.After further examination by a doctor at the WDRH upon arrival, they were admitted.Fredericks is said to have sustained a fractured left leg while Mondesir is suffering from fractured ribs. In addition to those injuries, the duo also sustained several lacerations about their bodies.According to a Police source, a breathalyser test was conducted on Ramnarine, and results revealed that the minibus driver had alcohol in his system that is above the prescribed legal limit.His vehicle, along with the motorcycle, is impounded at the Leonora Police Station, WCD, as the investigation continues. (Kristen Macklingam)last_img read more

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Arcata coach Ryan Bisio’s position in jeopardy after school board vote

first_imgArcata >> Arcata High School head basketball coach Ryan Bisio may be out as the Tigers head coach after the Northern Humboldt Union School District’s Board of Trustees voted to leave him off the list of approved coaches for the upcoming winter sports season.Bisio, who has compiled a 68-19 record in three seasons as the Tigers head coach, was removed from the school’s ‘Changes of Certified and Classified Personnel’ list presented to the school board at a meeting on Tuesday at the McKinleyville …last_img

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A’s facing a dilemma with catchers this spring

first_img“We do like the platoon situation, it’s why we signed him,” A’s manager Bob Melvin said of Herrmann. … MESA, Ariz. — A competition is brewing at the A’s catcher position this spring.Chris Herrmann was signed in December as a left-handed hitting catcher who was expected to serve as a complement to right-handed hitting Josh Phegley in a platoon role. But the arrival of another right-handed hitting catcher in Nick Hundley on a minor league deal just as the A’s broke camp is complicating things.last_img

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Local heroines in the spotlight

first_imgThe graves of Helen Joseph, Lilian Ngoyiand Charlotte Maxeke will be declared national heritage sites by the departmentof arts and culture and the South AfricanHeritage Resources Agency on 20 August (Image: Nosimilo Ramela) MEDIA CONTACTS • Mack Lewele Department of Arts and Culture +27 82 450 5076 or +27 012 441 3083 RELATED ARTICLES • African women forging ahead in IT • SA women take the lead • SA women in the spotlight • Media awards for SA womenNosimilo RamelaIn celebration of Women’s Month in South Africa, the Department of Arts and Culture has launched a number of initiatives to honour female icons who’ve helped shape a more democratic society.Women’s Month is always held in August, with a public holiday on the ninth, to pay tribute to the more than 20 000 South African women who marched to the Union Buildings on that date in 1956 to protest against unjust pass laws, which enforced racial segregation.This peaceful demonstration was led by apartheid activists Lilian Ngoyi, Helen Joseph, Rahima Moosa and Sophie de Bruyn, and put women at the forefront of the political struggle.“Through their actions, they helped to lay the foundations for the building of a non-racial, non-sexist and democratic country,” said Minister of Arts and Culture Lulu Xingwana.She was speaking at her department’s Women’s Month launch at The Rosebank Hotel, Johannesburg, on 3 August 2010.Women have always played a central role in the socio-economic development of society, but their roles have often been overlooked, the minister said.“The programmes we are launching are to declare that women also made a contribution.”Graves become heritage sitesThe department and the South African Heritage Resources Agency will declare the graves of Joseph, Ngoyi and fellow freedom fighter Charlotte Maxeke national heritage sites on 20 August. “This is part of the national legacy projects which honour the forgotten and unsung heroines of the liberation struggle,” said Xingwana.The ceremony will take place in Soweto, where Joseph and Ngoyi are buried. “The programme will include a church service, speeches and the unveiling of site plaques,” the minister added.Dulcie September Memorial LectureA day before this ceremony on 19 August, the department, in partnership with the University of the Western Cape, will host a memorial lecture in the name of another anti-apartheid icon, Dulcie September, who was assassinated in 1988 while in exile in France.“Through this lecture the government acknowledges her contribution towards the liberation of the people of South Africa,” said Xingwana.The lecture will be delivered by award-winning British writer and editor Margaret Busby, with guest speakers Dr John Daniel – a researcher from the Human Sciences Research Council – and South African ambassador to the US, Barbra Masekela.Xingwana said this lecture was a symbol of her department’s commitment to women: “We constantly see lectures recognising male heroes and rarely do we have lectures about women.”Women writers unite“We will host a women writers’ symposium under the theme Women’s words, African worlds, renewing a dialogue between African women writers and women of African descent,” Xingwana said at the launch.The gathering will take place from 25 to 26 August at two Johannesburg venues: the Windybrow Theatre and Museum Africa. It will focus on fiction and non-fiction writing, journalism and the formation of an African Women Writers’ Network.Invited writers who will participate in the symposium include Tsitsi Dangarembga of Zimbabwe, Lola Shoneyin of Nigeria, Shailja Patel of Kenya and Lauretta Ngcobo, Miriam Tlali and Elinor Sisulu from South Africa.Sarah Bartmann rememberedTo reaffirm the often marginalised history, heritage and culture of the indigenous Khoi and San Bushmen of South Africa, the department will unveil the design for the Sarah Bartmann Centre of Remembrance, on the 22 August in Hankey, in the Eastern Cape province.Bartmann was a Khoi slave who was lured to London in 1810 and put on show as a “scientific curiosity”, owing to her physical features that were perceived by Europeans as “freakish”.She was later moved to Paris, where she was exhibited by an animal trainer in severely degrading displays intended for public amusement.She died in France in December 1815 and her remains were exhibited in a museum there until 1974.Years later, then president of South Africa Nelson Mandela requested that her remains be returned. This was done, and on 9 August 2002 Bartmann was buried in Hankey, the town of her birth.SA women shine at Shanghai expoThe country is currently taking part in the six-month-long Shanghai World Exhibition, for which the Department of Arts and Culture developed a programme to showcase the talents of South African women artists and performers.Each country participating in the Shanghai Exhibition is allocated a national day – and South Africa has chosen 9 August. “As part of celebrating our national day we will hoist the South African flag,” said Xingwana.Prominent South African female musicians, including Debra Fraser, Loveline Madumo, Sibongile Khumalo and Thandiswa Mazwai, will perform at the event.Beyond Women’s MonthAll programmes launched will be sustained beyond the month of August to help empower and inspire women, Xingwana said, adding that “freedom is not complete until women are free from all forms of abuse, poverty, and deprivation”.last_img read more

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Spredfast: How to Keep Up with the Social Web

first_imgRelated Posts That brings us to the last point – tracking and metrics. As of now, the system has already incorporated Google Analytics and is looking to work with Armature and WebTrends in the near future. But even now, it offers full reports on the effectiveness of your message. Campaigns are judged with three primary measurements – engagement, reach and activity. Put simply, these look at your interaction with your audience, the size of your audience, and how much you are pushing your message out onto the Web. Beyond these basic ranks, however, the system will give you detailed reports showing you how many “likes” you’ve gotten on a specific message on Facebook, or how many times a tweet has been retweeted. But that’s even just the tip of the metrics iceberg, as it keeps stats on each individual tweet, blog post, status update, what have you offering a full variety of data on click-throughs, impressions and whatever you would expect from a traffic analytics service. Tags:#news#web Starting today, the system is available on a monthly basis starting at $50 per month, per campaign, for a single user. The “Enterprise” plan, at $100 per month, per campaign, allows for multiple users. Social Agency plans to announce another plan in February that will allow for unlimited users and campaigns, but the details for that are not yet available. For a program with this many features at launch, we only see it getting better. Why Tech Companies Need Simpler Terms of Servic… When we first looked at Spredfast, the social campaign management tool launched today by Austin, Texas-based Social Agency, we thought it looked like a less-flashy version of TweetDeck. Our first question to co-founders Kenneth Cho and Scott McCaskill was actually how the two programs differed and they took it in stride, given how far off the question really was. With big names like IBM, AOL, Cisco, Intel and Porter Novelli using the service, you better bet it does more than manage a handful of social networking accounts and microblogging services.Spredfast wants to be a new player in the field of social campaign management, and it is set to compete with other big names like Objective Marketer and Radian6.The entirely Web-based application is a full set of tools to not only manage and measure the message a company sends out among various social media, but also track the people who send the message. With fully customizable user roles and permissions, Spredfast looks like a great way to target multiple audiences on the Web from multiple directions. We think the key word here is multiple. This is a tool that can make a single person appear, to the average Web user, to be an entire community of people talking about your product.Like single-user tools of this variety, such as TweetDeck or Ping.fm, Spredfast is set up to work with any of the standard social networks. In addition to that, however, it will work with a number of content management systems, from Drupal and WordPress to Movable Type. And with its user-role management, you can not only say who can post to what, but whether or not it can go live or needs to be reviewed by another user before being published. On-site scheduling and voice management allows a single tweet to be sent out and then be retweeted, in slightly altered forms, by any number of other accounts over time, creating the illusion of a discussion. We may never trust what we see people talking about on the web again.In addition to multiple users, roles, networks and blogging platforms, Spredfast is also set to handle multiple campaigns. So, if ReadWriteWeb were to suddenly start using the system, for example, we could track activity for ReadWriteStart and ReadWriteEnterprise separately. center_img Top Reasons to Go With Managed WordPress Hosting mike melanson A Web Developer’s New Best Friend is the AI Wai… 8 Best WordPress Hosting Solutions on the Marketlast_img read more

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Solidarity aside Costa Rica has little to offer Venezuela

first_imgRelated posts:Exiles in Costa Rica denounce alleged Venezuelan kidnapping plot Costa Rican lawmakers urge political asylum for Venezuela opposition Condoms at $755 show Venezuela reeling from plunging oil prices Venezuela opposition controls legislature minus supermajority – for now Costa Rica, president pro tempore of the Community of Latin American and Caribbean States (CELAC), released a statement Monday rejecting the recent violence in Venezuela and urging the government to comply with the rule of law and ensure the safety of its citizens.A section of the brief statement read:“The countries of CELAC express their solidarity with the people of the Bolivarian Republic of Venezuela and encourage their government to continue its efforts to foster a dialogue between all the political forces in the country for the sake of peace and national unity that the Venezuelan people require to continue their march toward progress and well being.”Besides the declaration, however, there is little more Costa Rica can do with its rotating presidency of the community of 33 nations, according to Foreign Minister Enrique Castillo.“Costa Rica can not unilaterally speak out in the name of CELAC without prior consensus,” the foreign minister said. “CELAC is not an organization, it’s a mechanism for dialogue. A meeting of foreign ministers would not be possible in a few days in the heat of the moment when events are changing every day.” Hundreds of Venezuelans living in Costa Rica arrived in front of San José’s National Theater on Feb. 16 to show solidarity with protesters in their home country. (Courtesy of José Pablo Ávila)Venezuelan opposition leader Leopoldo López surrendered to authorities Tuesday after Venezuelan President Nicolás Maduro ordered his arrest, accusing López of inciting the violence that claimed the lives of three demonstrators on Feb. 12.“We trust that they will be able to make an effort to sit down at the table and talk. The only ones who will suffer from the lack of dialogue and the lack of tolerance will be the people of Venezuela,” Costa Rican President Laura Chinchilla said on Tuesday during her weekly address.Castillo said that political asylum for Venezuelan opposition leaders was a possibility, but the government of Costa Rica has not received any formal requests for safe haven.Meanwhile, former president and Nobel Peace Prize winner Oscar Arias accepted an invitation Tuesday to serve as an international observer in Venezuela. Hundreds of Venezuelans living in Costa Rica arrived in front of San José’s National Theater on Feb. 16 to show solidarity with protesters in their home country. (Courtesy of José Pablo Ávila) Facebook Commentslast_img read more

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